BK-Gentier-48E Real-time PCR System
BK-Gentier-48E Real-time PCR System
Features
1. DESIGN FOR FLEXIBLE USE
Standard with 7" LCD touch screen, functions quickly, accessible through simple, one-touch commands.
Wizard operation to lock and unlock the transport locker.
Message alarm indicate the status of the machine.
USB port update software and firmware.
Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.
2. FAST AND UNIFORM HEATING SYSTEM
Concealed lid with constant temperature and isopiestic pressure.
Electrical controlled elevating & automatic locked platform.
Thermo block is based on improved heat pipe technology, leading to extraordinary lintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
Highly efficient PID&DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking.
3. HIGH SENSITIVE AND PRECISE OPTICAL SYSTEM
High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could be from 101 to 1010 copies.
Fast and precise mechanical scanning structure ensures the illumination uniformity of 96 wells, eliminate edge effects, no need for ROX calibration.
Fluorescence excitation by a high-intensity and long-life LED. Bulb Luminance will not attenuation over time, free maintenance optical system through the entire life.
Professional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.
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CH1 |
CH2 |
CH3 |
CH4 |
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FAM SYBR Green I SYTO 9 Eva Green LC Green |
HEX VIC TET JOE |
Texas Red ROX |
Cy5 |
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BK-Gentier 48E 465 /510 (FAM,SYBR Green l,SYTO9,Eva Green,LC Green) 527/ 563 (HEX,VIC,TET, JOE) 580 /616 (ROX,Texas Red) 632/664( Cy5) Channels are same with Roche Light Cycler 96, Agilent Mx3000P |
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EASY-TO-USE SOFTWARE
Friendly interface and clearly functional modules design, first-time users can easily start a new experiment.
All experiment data and setting options are shown conveniently on a single interface.
Instrument Management allow you managing all the Gentiers in the WLAN.
More than thousands results can be stored in device.
USB or WLAN easily transfer experiments data anywhere you needed.
Multi-options for experiments: New, New for Existed, Open data file, Double click data file.
User can define the experiments name for documentation
User can define the data storage directory themself
Pre-installed templates can help user more easier to edit the protocals.
RUN MONITORING
All running information in one screen, intuitively and simply
User can define each model to see what they like on the screen: Real-time Fluorescence ,Heat map, Run inf。,Sample Setting, Sample info...
Run the experiment first by device, later edit the sample setting in PC, when PC connected to machine, setting upload to machine automatically.
After experiment, data download to PC automatically.
DATA ANALYSIS
Optional baseline setting: Automatic or Manual.
Multi-option analytical method for different requirements: Automatic or Manual threshold method, normalization method.
Reference Dye analysis.
DATA ANALYSIS-OTHERS
A Statistics analysis: Replicate,Gene types.
A Heating map options: Ct, Concentration, Florescence.
A LIS connectivity, can export csv, Excel and Txt format.
A Row data and results can export in Excel format.
REPORT
Unique designed universal report(Research and Clinical) can be customized according to the requirements, report review and print function help clinical documentation as needed.
QUALITY DATA
Specification Table
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Model |
BK-Gentier 48E |
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Thermal Block |
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Sample capacity(wells) |
48 |
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Reaction Volume |
5-100 μL |
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Consumables |
0.2ml 8-strip tubes ,0.2ml PCR single tube(Optical flat cap,clear tube) |
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Temperature range |
0°C-100°C |
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Heating/cooling method |
Peltier |
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Max heating rate |
8.0°C/sec |
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Average heating rate |
6.2°C/sec |
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Max cooling rate |
6.2°C/sec |
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Average cooling rate |
4.5°C/sec |
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Temperature accuracy |
±0.1℃ |
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Temperature uniformity |
±0.2℃ |
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Gradient Range |
1°C-40°C |
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Gradient block |
8 row |
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Special temperature portocol |
Gradient PCR,Long PCR,Touch Down PCR |
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Heat Lid |
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Temperature range |
Room Temperature–110°C |
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Optical System |
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Excitation Source |
4LEDs |
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Detector |
Photodiode |
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Detection Position |
Excitation and scan from lateral |
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Detection method |
4 channels scanning at the same time, no edge effect. |
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Detection time |
2 seconds for 48 wells for all channels |
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Range of |
1 465 /510 (FAM,SYBR Green I，SYTO9，EvaGreen，LC Green) |
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Probe |
Taqman Probe,Molecular beacons probe，Scorpion probe |
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Multiplexing |
Up to 4 targets |
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Fluorescence Linearity |
r≥0.990 |
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Fluorescence Dynamic Range |
Adjustable |
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Performance |
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Sample Linearity |
/r/≥0.999 |
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Sample repeatability |
Ct value CV≤0.5% |
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Sample Dynamic Range |
1-1010 copies |
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Software Funtions |
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Data Analysis modes |
Qualitative analysis, Absolute quantification, Relative quantification, Genotyping analysis, Endpoint analysis， High Resolution Melting |
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Control modes |
1.7 touch screen |
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Data Stroage |
Upload and download through USB disk,1000 results can be stored in machine |
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Power failure protection |
Automaticly start running experiments after power supply, no need wait PC software |
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Customize Report |
Templetes reserved ,report can customized |
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Administration Management |
Administrator can set funtions limts for users |
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Fault Management |
Fault report and analysis, solution instruction |
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LIS connection |
CSV,Excel,TXT format data output,open port for LIS connection |
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Others |
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PC operating system |
Win 7, Win 10 |
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Communication Port |
1 ethernet and 2 USB |
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Footprint(WxDxH) |
260mm X 400mm X 2605mm |
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Weight |
11kg |
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Power usage |
AC 100 to 125 V / 200 to 240 V (50/60 Hz). |
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power consumption |
600VA |
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Work environment |
Tempereture:10°C~30°C Humidity:20%~85% |
